Rising methane gas bubbles form massive hydrate layers at the seafloor

Extensive methane hydrate layers are formed in the near-surface sediments of the Cascadia margin. An undissociated section of such a layer was recovered at the base of a gravity core (i.e. at a sediment depth of 120 cm) at the southern summit of Hydrate Ridge. As a result of salt exclusion during methane hydrate formation, the associated pore waters show a highly elevated chloride concentration of 809 mM. In comparison, the average background value is 543 mM.A simple transport-reaction model was developed to reproduce the Cl⁻ observations and quantify processes such as hydrate formation, methane demand, and fluid flow. From this first field observation of a positive Cl⁻ anomaly, high hydrate formation rates (0.15-1.08 mol cm⁻² a⁻¹) were calculated. Our model results also suggest that the fluid flow rate at the Cascadia accretionary margin is constrained to 45-300 cm a⁻¹. The amount of methane needed to build up enough methane hydrate to produce the observed chloride enrichment exceeds the methane solubility in pore water. Thus, most of the gas hydrate was most likely formed from ascending methane gas bubbles rather than solely from CH₄ dissolved in the pore water.     

Neogene magnetic polarity stratigraphy of some fluviatile Siwalik sections, Nepal

The magnetostratigraphy of five new sections through the Neogene Siwalik Group of Nepal is presented. Rock magnetic experiments and detailed thermal demagnetization experiments prove that haematite is the carrier of a primary DRM or a PDRM. After stepwise thermal demagnetization of specimens from all sections, directions of characteristic remanent magnetization were obtained. The results show a positive reversal test and exhibit inclination errors of about 20. Due to gaps in exposures and extremely variable demagnetization behaviour of the haematite-bearing sediments, the resulting polarity sequences are poorly defined for some parts of the sections. However, double-sampled parts of some sections yield similar results. A correlation with the Surai Khola section (Appel, Rösler & Corvinus 1991) is proposed, based on rock magnetic parameters. AMS results from three of the sections prove the existence of primary sedimentary magnetic fabrics in these sections with systematic orientations of minimum and maximum susceptibility axes. Correlation with a standard polarity timescale indicates that the ages of all new sections lie within the age limits of the Surai Khola section.     

Morphologically based sampling designs for zoned orthopyroxenes

Compositional data of orthopyroxene crystals obtained by the electron microprobe may be biased unless zoning morphologies are taken into account in the sampling design. Statistical models (predictive or interpretative) based on such data are open to question because the input data, such as correlations, are spurious. In an attempt to propose sampling designs two established crystal zoning morphologies are considered which are (a) the concentric shell-type and (b) sectoral zonations. These zonational types lend themselves well to statistical population modeling and graphical interpretation provided assumptions of chemical invariance hold or are approximated by a stratified sampling design. The statistical designs may be regarded as limiting cases in the sense that relaxations of certain assumptions lead to other population models. Hypersthene crystals from a central portion of the mafic norite formation at the Strathcona Mine, Sudbury irruptive, are examined for zonation. It appears that the trace elements have a different zoning morphology than the major elements of hypersthene.     

Effects of Local Shoreline and Subestuary Watershed Condition on Waterbird Community Integrity: Influences of Geospatial Scale and Season in the Chesapeake Bay

In many coastal regions throughout the world, there is increasing pressure to harden shorelines to protect human infrastructures against sea level rise, storm surge, and erosion. This study examines waterbird community integrity in relation to shoreline hardening and land use characteristics at three geospatial scales: (1) the shoreline scale characterized by seven shoreline types: bulkhead, riprap, developed, natural marsh, Phragmites-dominated marsh, sandy beach, and forest; (2) the local subestuary landscape scale including land up to 500 m inland of the shoreline; and (3) the watershed scale >500 m from the shoreline. From 2010 to 2014, we conducted waterbird surveys along the shoreline and open water within 21 subestuaries throughout the Chesapeake Bay during two seasons to encompass post-breeding shorebirds and colonial waterbirds in late summer and migrating and wintering waterfowl in late fall. We employed an Index of Waterbird Community Integrity (IWCI) derived from mean abundance of individual waterbird species and scores of six key species attributes describing each species’ sensitivity to human disturbance, and then used this index to characterize communities in each subestuary and season. IWCI scores ranged from 14.3 to 19.7. Multivariate regression model selection showed that the local shoreline scale had the strongest influence on IWCI scores. At this scale, percent coverage of bulkhead and Phragmites along shorelines were the strongest predictors of IWCI, both with negative relationships. Recursive partitioning revealed that when subestuary shoreline coverage exceeded thresholds of approximately 5% Phragmites or 8% bulkhead, IWCI scores decreased. Our results indicate that development at the shoreline scale has an important effect on waterbird community integrity, and that shoreline hardening and invasive Phragmites each have a negative effect on waterbirds using subestuarine systems.     

Fluid venting activity on the Costa Rica margin: new results from authigenic carbonates

Carbonate precipitates on mounds and along tectonic scarps off the Costa Rica margin are manifestations of subduction-induced dewatering. The long-term dewatering history is recorded in mineralogical, petrological and isotope signals of carbonates recovered from these sites. The carbonates are strongly depleted in ¹³C (–11 to –53 PDB) and enriched in ¹⁸O (+4 to +8 PDB). Thermogenic methane and biogenic methane were identified as sources of the carbon. Chemoherm carbonates and seepage-associated carbonates formed in a focused flow regime have lighter ¹³C values, while others formed in a more diffusive flow regime have slightly enriched C isotope values. Three fluid components were inferred based on the calculation of equilibrium ¹⁸O: clay dehydration water, gas hydrate water and seawater. Calculated equilibrium ¹⁸O values of carbonates from different down-core depths as well as from different precipitation stages show that the ¹⁸O of the precipitating fluid is progressively depleted with time. Dolostones showing a methane-C source and a well constrained O-isotope signature are thought to have formed at depth in the sediment and subsequently became exhumed. Glauconitic sandstones cemented by methane-derived carbonate provide evidence that fluid and solid material have been expelled by the mud volcano.     

Differential developmental toxicity of naphthoic acid isomers in medaka (Oryzias latipes) embryos

Polycyclic aromatic hydrocarbons (PAHs) are widespread persistent pollutants that readily undergo biotic and abiotic conversion to numerous transformation products in rivers, lakes and estuarine sediments. Here we characterize the developmental toxicity of four PAH transformation products each structural isomers of hydroxynaphthoic acid: 1H2NA, 2H1NA, 2H3NA, and 6H2NA. Medaka fish (Oryzias latipes) embryos and eleutheroembryos were used to determine toxicity. A 96-well micro-plate format was used to establish a robust, statistically significant platform for assessment of early life stages. Individual naphthoic acid isomers demonstrated a rank order of toxicity with 1H2NA>2H1NA>2H3NA>6H2NA being more toxic. Abnormalities of circulatory system were most pronounced including pericardial edema and tube heart. To determine if HNA isomers were AhR ligands, spatial-temporal expression and activity of CYP1A was measured via in vivo EROD assessments. qPCR measurement of CYP1A induction proved different between isomers dosed at respective concentrations affecting 50% of exposed individuals (EC50s). In vitro, all ANH isomers transactivated mouse AhR using a medaka CYP1A promoter specific reporter assay. Circulatory abnormalities followed P450 induction and response was consistent with PAH toxicity. A 96-well micro-plates proved suitable as exposure chambers and provided statistically sound evaluations as well as efficient toxicity screens. Our results demonstrate the use of medaka embryos for toxicity analysis thereby achieving REACH objectives for the reduction of adult animal testing in toxicity evaluations.